Assembly of proteasome subunits into non-canonical complexes in vivo

Proteasomes exist in all domains of life. In general, they are comprised of a compartmentalized protease whose activity is modulated by one or more regulatory complexes with which it interacts. The quaternary structure of this compartmentalized protease, called the 20S proteasome, is absolutely conserved and consists of four heptameric rings stacked coaxially. The rings are made of structurally related α and β subunits. In eukaryotes, assembly factors chaperone the α and β subunits during 20S biogenesis. Here we demonstrate that proteasome subunits can assemble into structures other than the canonical 20S proteasome in vivo. Specifically, the yeast α4 subunit forms high molecular weight complexes whose abundance increases when proteasome function is compromised. Results from a disulfide crosslinking approach are consistent with these complexes being ring-shaped. Though several eukaryotic α subunits can form rings when expressed recombinantly in bacteria, this is the first evidence that such non-canonical complexes exist in vivo

Instrument manual for the retarding ion mass spectrometer on Dynamics Explorer-1

The retarding ion mass spectrometer (RIMS) for Dynamics Explorer-1 is an instrument designed to measure the details of the thermal plasma distribution. It combines the ion temperature determining capability of the retarding potential analyzer with the compositional capabilities of the mass spectrometer and adds multiple sensor heads to sample all directions relative to the spacecraft ram direction. This manual provides a functional description of the RIMS, the instrument calibration, and a description of the commands which can be stored in the instrument logic to control its operation

Molecular chaperones of the Hsp70 family assist in the assembly of 20S proteasomes

The eukaryotic 26S proteasome is a large protease comprised of two major sub assemblies, the 20S proteasome, or core particle (CP), and the 19S regulatory particle (RP). Assembly of the CP and RP is assisted by an expanding list of dedicated assembly factors. For the CP, this includes Ump1 and the heterodimeric Pba1–Pba2 and Pba3–Pba4 proteins. It is not known how many additional proteins that assist in proteasome biogenesis remain to be discovered. Here, we demonstrate that two members of the Hsp70 family in yeast, Ssa1 and Ssa2, play a direct role in CP assembly. Ssa1 and Ssa2 interact genetically and physically with proteasomal components. Specifically, they associate tightly with known CP assembly intermediates, but not with fully assembled CP, through an extensive purification protocol. And, in yeast lacking both Ssa1 and Ssa2, specific defects in CP assembly are observed

Pancharatnam-Berry phase in condensate of indirect excitons

We report on the observation of the Pancharatnam-Berry phase in a condensate of indirect excitons (IXs) in a GaAs coupled quantum well structure. The Pancharatnam-Berry phase leads to phase shifts of interference fringes in IX interference patterns. Correlations are found between the phase shifts, polarization pattern of IX emission, and onset of IX spontaneous coherence. The Pancharatnam-Berry phase is acquired due to coherent spin precession in IX condensate. The effect of the Pancharatnam-Berry phase on the IX phase pattern is described in terms of an associated momentum.Comment: 6 pages, 5 figures + 2 pages supplemental material, 3 supplemental figure

Space capsule Patent

Manned space capsule configuration for orbital flight and atmospheric reentr

The retarding ion mass spectrometer on dynamics Explorer-A

An instrument designed to measure the details of the thermal plasma distribution combines the ion temperature-determining capability of the retarding potential analyzer with the compositional capabilities of the mass spectrometer and adds multiple sensor heads to sample all directions relative to the spacecraft ram directions. The retarding ion mass spectrometer, its operational modes and calibration are described as well as the data reduction plan, and the anticipated results

Covering Partial Cubes with Zones

A partial cube is a graph having an isometric embedding in a hypercube. Partial cubes are characterized by a natural equivalence relation on the edges, whose classes are called zones. The number of zones determines the minimal dimension of a hypercube in which the graph can be embedded. We consider the problem of covering the vertices of a partial cube with the minimum number of zones. The problem admits several special cases, among which are the problem of covering the cells of a line arrangement with a minimum number of lines, and the problem of finding a minimum-size fibre in a bipartite poset. For several such special cases, we give upper and lower bounds on the minimum size of a covering by zones. We also consider the computational complexity of those problems, and establish some hardness results

Water waves generated by a moving bottom

Tsunamis are often generated by a moving sea bottom. This paper deals with the case where the tsunami source is an earthquake. The linearized water-wave equations are solved analytically for various sea bottom motions. Numerical results based on the analytical solutions are shown for the free-surface profiles, the horizontal and vertical velocities as well as the bottom pressure.Comment: 41 pages, 13 figures. Accepted for publication in a book: "Tsunami and Nonlinear Waves", Kundu, Anjan (Editor), Springer 2007, Approx. 325 p., 170 illus., Hardcover, ISBN: 978-3-540-71255-8, available: May 200

Coherence Length of Cold Exciton Gases in Coupled Quantum Wells

A Mach-Zehnder interferometer with spatial and spectral resolution was used to probe spontaneous coherence in cold exciton gases, which are implemented experimentally in the ring of indirect excitons in coupled quantum wells. A strong enhancement of the exciton coherence length is observed at temperatures below a few Kelvin. The increase of the coherence length is correlated with the macroscopic spatial ordering of excitons.Comment: 5 pages, 3 figure